Rpi-blb2-Mediated Hypersensitive Cell Death Caused by Phytophthora infestans AVRblb2 Requires SGT1, but not EDS1, NDR1, Salicylic Acid-, Jasmonic Acid-, or Ethylene-Mediated Signaling.
Identifieur interne : 000F90 ( Main/Exploration ); précédent : 000F89; suivant : 000F91Rpi-blb2-Mediated Hypersensitive Cell Death Caused by Phytophthora infestans AVRblb2 Requires SGT1, but not EDS1, NDR1, Salicylic Acid-, Jasmonic Acid-, or Ethylene-Mediated Signaling.
Auteurs : Sang-Keun Oh [Corée du Sud] ; Suk-Yoon Kwon [Corée du Sud] ; Doil Choi [Corée du Sud]Source :
- The plant pathology journal [ 1598-2254 ] ; 2014.
Abstract
Potato Rpi-blb2 encodes a protein with a coiled-coil-nucleotide binding site and leucine-rich repeat (CC-NBS-LRR) motif that recognizes the Phytophthora infestans AVRblb2 effector and triggers hypersensitive cell death (HCD). To better understand the components required for Rpi-blb2-mediated HCD in plants, we used virus-induced gene silencing to repress candidate genes in Rpi-blb2-transgenic Nicotiana benthamiana plants and assayed the plants for AVRblb2 effector. Rpi-blb2 triggers HCD through NbSGT1-mediated pathways, but not NbEDS1- or NbNDR1-mediated pathways. In addition, the role of salicylic acid (SA), jasmonic acid (JA), and ethylene (ET) in Rpi-blb2-mediated HCD were analyzed by monitoring of the responses of NbICS1-, NbCOI1-, or NbEIN2-silenced or Rpi-blb2::NahG-transgenic plants. Rpi-blb2-mediated HCD in response to AVRblb2 was not associated with SA accumulation. Thus, SA affects Rpi-blb2-mediated resistance against P. infestans, but not Rpi-blb2-mediated HCD in response to AVRblb2. Additionally, JA and ET signaling were not required for Rpi-blb2-mediated HCD in N. benthamiana. Taken together, these findings suggest that NbSGT1 is a unique positive regulator of Rpi-blb2-mediated HCD in response to AVRblb2, but EDS1, NDR1, SA, JA, and ET are not required.
DOI: 10.5423/PPJ.OA.03.2014.0027
PubMed: 25289011
PubMed Central: PMC4181110
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Rpi-blb2-Mediated Hypersensitive Cell Death Caused by Phytophthora infestans AVRblb2 Requires SGT1, but not EDS1, NDR1, Salicylic Acid-, Jasmonic Acid-, or Ethylene-Mediated Signaling.</title><author><name sortKey="Oh, Sang Keun" sort="Oh, Sang Keun" uniqKey="Oh S" first="Sang-Keun" last="Oh">Sang-Keun Oh</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant Science, Plant Genomics and Breeding Institute, Institute of Agricultural Biotechnology, Seoul National University, Seou1 151-742, Korea ; Korea Research Institute of Bioscience & Biotechnology, Daejeon 305-806, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Plant Science, Plant Genomics and Breeding Institute, Institute of Agricultural Biotechnology, Seoul National University, Seou1 151-742, Korea ; Korea Research Institute of Bioscience & Biotechnology, Daejeon 305-806</wicri:regionArea><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author><author><name sortKey="Kwon, Suk Yoon" sort="Kwon, Suk Yoon" uniqKey="Kwon S" first="Suk-Yoon" last="Kwon">Suk-Yoon Kwon</name><affiliation wicri:level="1"><nlm:affiliation>Korea Research Institute of Bioscience & Biotechnology, Daejeon 305-806, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Korea Research Institute of Bioscience & Biotechnology, Daejeon 305-806</wicri:regionArea><wicri:noRegion>Daejeon 305-806</wicri:noRegion></affiliation></author><author><name sortKey="Choi, Doil" sort="Choi, Doil" uniqKey="Choi D" first="Doil" last="Choi">Doil Choi</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant Science, Plant Genomics and Breeding Institute, Institute of Agricultural Biotechnology, Seoul National University, Seou1 151-742, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Plant Science, Plant Genomics and Breeding Institute, Institute of Agricultural Biotechnology, Seoul National University, Seou1 151-742</wicri:regionArea><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:25289011</idno><idno type="pmid">25289011</idno><idno type="doi">10.5423/PPJ.OA.03.2014.0027</idno><idno type="pmc">PMC4181110</idno><idno type="wicri:Area/Main/Corpus">000F55</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000F55</idno><idno type="wicri:Area/Main/Curation">000F55</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000F55</idno><idno type="wicri:Area/Main/Exploration">000F55</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Rpi-blb2-Mediated Hypersensitive Cell Death Caused by Phytophthora infestans AVRblb2 Requires SGT1, but not EDS1, NDR1, Salicylic Acid-, Jasmonic Acid-, or Ethylene-Mediated Signaling.</title><author><name sortKey="Oh, Sang Keun" sort="Oh, Sang Keun" uniqKey="Oh S" first="Sang-Keun" last="Oh">Sang-Keun Oh</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant Science, Plant Genomics and Breeding Institute, Institute of Agricultural Biotechnology, Seoul National University, Seou1 151-742, Korea ; Korea Research Institute of Bioscience & Biotechnology, Daejeon 305-806, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Plant Science, Plant Genomics and Breeding Institute, Institute of Agricultural Biotechnology, Seoul National University, Seou1 151-742, Korea ; Korea Research Institute of Bioscience & Biotechnology, Daejeon 305-806</wicri:regionArea><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author><author><name sortKey="Kwon, Suk Yoon" sort="Kwon, Suk Yoon" uniqKey="Kwon S" first="Suk-Yoon" last="Kwon">Suk-Yoon Kwon</name><affiliation wicri:level="1"><nlm:affiliation>Korea Research Institute of Bioscience & Biotechnology, Daejeon 305-806, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Korea Research Institute of Bioscience & Biotechnology, Daejeon 305-806</wicri:regionArea><wicri:noRegion>Daejeon 305-806</wicri:noRegion></affiliation></author><author><name sortKey="Choi, Doil" sort="Choi, Doil" uniqKey="Choi D" first="Doil" last="Choi">Doil Choi</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant Science, Plant Genomics and Breeding Institute, Institute of Agricultural Biotechnology, Seoul National University, Seou1 151-742, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Plant Science, Plant Genomics and Breeding Institute, Institute of Agricultural Biotechnology, Seoul National University, Seou1 151-742</wicri:regionArea><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author></analytic><series><title level="j">The plant pathology journal</title><idno type="ISSN">1598-2254</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Potato Rpi-blb2 encodes a protein with a coiled-coil-nucleotide binding site and leucine-rich repeat (CC-NBS-LRR) motif that recognizes the Phytophthora infestans AVRblb2 effector and triggers hypersensitive cell death (HCD). To better understand the components required for Rpi-blb2-mediated HCD in plants, we used virus-induced gene silencing to repress candidate genes in Rpi-blb2-transgenic Nicotiana benthamiana plants and assayed the plants for AVRblb2 effector. Rpi-blb2 triggers HCD through NbSGT1-mediated pathways, but not NbEDS1- or NbNDR1-mediated pathways. In addition, the role of salicylic acid (SA), jasmonic acid (JA), and ethylene (ET) in Rpi-blb2-mediated HCD were analyzed by monitoring of the responses of NbICS1-, NbCOI1-, or NbEIN2-silenced or Rpi-blb2::NahG-transgenic plants. Rpi-blb2-mediated HCD in response to AVRblb2 was not associated with SA accumulation. Thus, SA affects Rpi-blb2-mediated resistance against P. infestans, but not Rpi-blb2-mediated HCD in response to AVRblb2. Additionally, JA and ET signaling were not required for Rpi-blb2-mediated HCD in N. benthamiana. Taken together, these findings suggest that NbSGT1 is a unique positive regulator of Rpi-blb2-mediated HCD in response to AVRblb2, but EDS1, NDR1, SA, JA, and ET are not required. </div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">25289011</PMID><DateCompleted><Year>2014</Year><Month>10</Month><Day>07</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>29</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1598-2254</ISSN><JournalIssue CitedMedium="Print"><Volume>30</Volume><Issue>3</Issue><PubDate><Year>2014</Year><Month>Sep</Month></PubDate></JournalIssue><Title>The plant pathology journal</Title><ISOAbbreviation>Plant Pathol J</ISOAbbreviation></Journal><ArticleTitle>Rpi-blb2-Mediated Hypersensitive Cell Death Caused by Phytophthora infestans AVRblb2 Requires SGT1, but not EDS1, NDR1, Salicylic Acid-, Jasmonic Acid-, or Ethylene-Mediated Signaling.</ArticleTitle><Pagination><MedlinePgn>254-60</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.5423/PPJ.OA.03.2014.0027</ELocationID><Abstract><AbstractText>Potato Rpi-blb2 encodes a protein with a coiled-coil-nucleotide binding site and leucine-rich repeat (CC-NBS-LRR) motif that recognizes the Phytophthora infestans AVRblb2 effector and triggers hypersensitive cell death (HCD). To better understand the components required for Rpi-blb2-mediated HCD in plants, we used virus-induced gene silencing to repress candidate genes in Rpi-blb2-transgenic Nicotiana benthamiana plants and assayed the plants for AVRblb2 effector. Rpi-blb2 triggers HCD through NbSGT1-mediated pathways, but not NbEDS1- or NbNDR1-mediated pathways. In addition, the role of salicylic acid (SA), jasmonic acid (JA), and ethylene (ET) in Rpi-blb2-mediated HCD were analyzed by monitoring of the responses of NbICS1-, NbCOI1-, or NbEIN2-silenced or Rpi-blb2::NahG-transgenic plants. Rpi-blb2-mediated HCD in response to AVRblb2 was not associated with SA accumulation. Thus, SA affects Rpi-blb2-mediated resistance against P. infestans, but not Rpi-blb2-mediated HCD in response to AVRblb2. Additionally, JA and ET signaling were not required for Rpi-blb2-mediated HCD in N. benthamiana. Taken together, these findings suggest that NbSGT1 is a unique positive regulator of Rpi-blb2-mediated HCD in response to AVRblb2, but EDS1, NDR1, SA, JA, and ET are not required. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Oh</LastName><ForeName>Sang-Keun</ForeName><Initials>SK</Initials><AffiliationInfo><Affiliation>Department of Plant Science, Plant Genomics and Breeding Institute, Institute of Agricultural Biotechnology, Seoul National University, Seou1 151-742, Korea ; Korea Research Institute of Bioscience & Biotechnology, Daejeon 305-806, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kwon</LastName><ForeName>Suk-Yoon</ForeName><Initials>SY</Initials><AffiliationInfo><Affiliation>Korea Research Institute of Bioscience & Biotechnology, Daejeon 305-806, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Choi</LastName><ForeName>Doil</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Plant Science, Plant Genomics and Breeding Institute, Institute of Agricultural Biotechnology, Seoul National University, Seou1 151-742, Korea.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Korea (South)</Country><MedlineTA>Plant Pathol J</MedlineTA><NlmUniqueID>100966769</NlmUniqueID><ISSNLinking>1598-2254</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">AVRblb2 effector</Keyword><Keyword MajorTopicYN="N">EDS1</Keyword><Keyword MajorTopicYN="N">NDR1</Keyword><Keyword MajorTopicYN="N">Rpi-blb2</Keyword><Keyword MajorTopicYN="N">SGT1</Keyword><Keyword MajorTopicYN="N">hypersensitive cell 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